FOOD SYSTEMS AND BIOTECHNOLOGY OF FOOD AND BIOACTIVE SUBSTANCES
The article presents the results of the investigation of the quality indicators of flour from chickpeas, corn and rice. Gluten-free foods literally become a panacea to a healthy and happy life for people suffering from celiac disease, given that patients in the acute phase of the disease have a high risk of death compared to the general population. Complex therapy involves both drug treatment and a strict gluten-free diet. In addition, the popularity of gluten-free products has increased among people leading a healthy lifestyle, watching their diet, seeking to either reduce excess body weight or keep weight under control. Creating gluten-free products is a global trend. Bread is statistically the most consumed food product and the gluten-free bakery products market development industry has great potential.
The market analysis has shown that gluten-free bakery products are mainly produced from buckwheat, rice, oat or potato flour. Chickpea, rice and corn flour do not contain gluten and as a result can be used for dietary nutrition both for those suffering from celiac disease and for other categories of citizens.
The biochemical and physicochemical parameters of the studied types of flour have been considered. Taking into account that all the types of flour considered in the research are gluten-free, they can be recommended for the production of gluten-free bakery and flour confectionery products.
One of the segments of public catering market both abroad and in Russia is fast food products [1]. Restaurants of this format have a wide audience due to their loyal pricing policy, availability and constant new products [2]. In addition, the fast food industry, through various media and advertising, contributes to solving consumer problems related to lack of time, difficulty in preparation and ease of consumption. At the same time, fast food contributes to a decrease in the quality of nutrition and deterioration in the health of both young people and adults.
The goal of the research is to identify consumer preferences in the segment of semi-finished fish products, evaluate their quality and develop recipes for fish products for fast food restaurants.
The objectives of the research include marketing research of consumer preferences when choosing semi-finished fish products, assessing the quality of semi-finished fish products sold in retail trade and fast food establishments, and developing recipes for semi-finished fish products for the fast food industry. To conduct a social survey, Google-form has been used, organoleptic assessment has been carried out using 5-point grading scale; physicochemical indicators have been studied using standard methods.
Using the results of marketing research, it has been established that semi-finished fish products in the form of fish cutlets and sticks are in demand; consumers want to see new fish items on the menu of fast food restaurants. According to organoleptic assessment and physical and chemical research, it has been found that the best consumer organoleptic properties are found in fish cutlets from fast food restaurants «Vkusno i Tochka», «Burger King» and fish sticks sold in the «Perekrestok» chain of stores. According to physicochemical indicators, the studied samples of semi-finished fish products do not exceed 2.5% in the amount of table salt, 71% moisture and 37% fat.
As new items for the fast industry, recipes for semi-finished fish products were developed in the form of nuggets with the addition of processed cheese, pork fat and strips, from longitudinal strips of fish, breaded and deep-fried at a temperature of 180 ° C until golden brown.
There exists a problem of unbalanced nutrition in the modern world. Consumption of vegetables and fruits is mainly seasonal and they are not consumed by everyone in the daily diet in sufficient quantities to meet the daily requirement for nutrients. Snack products, including various bars, are becoming increasingly popular among the population of our country. Often there is an insufficient amount of vitamins and other micronutrients with such products that are so necessary to ensure the health of the human body. Insufficiency of these nutrients causes various diseases, the condition of the skin, hair, nails, and even mood worsens.
The goal of the research was to determine the amount of vitamins in a bar made from sunflower seeds with the addition of plant materials – crushed dried tomato fruits.
The objects of the study were a candy bar with the addition of rusk crumbs, containing 15% of dried tomatoes by weight of the finished product and a control sample – kozinak.
Tests were carried out on a Dionex Ultimate 3000 chromatograph using reverse phase chromatography on a Luna C18 column. This device is used to measure the content of a wide range of components in samples of substances and materials, solutions, food products and other objects of analysis.
It has been found that with the addition of a herbal additive and cracker crumbs to the recipe, the quanitative composition of vitamins A and E in the finished product increased compared to the control sample.
The results of the research can be used in the development of new food recipes.
The goal of the research is to evaluate the flavor and rheological compositions formed in the process of structuring gel-like solutions of various concentrations into individual processes of the technological scheme for the production of semi-finished products and complex interaction in the structure of the finished product, taking into account the use of gelling agents and dietary supplements. The article presents some features of the flow of physicochemical reactions that make it possible to describe the dynamics of the interaction of nutrients in the formed binary compositions of carbohydrate-fat structures of plant systems in the technological process.
Methods. To carry out the tests, the potentiometric method was used and the pH of each sample was determined using the Expert-001-3pH device. Determinations of sugars were carried out according to GOST 8756.13-87 using the permanganate method; dry substances according to GOST 31640-2012; organoleptic assessment according to GOST ISO 6658–2015. Samples of semi-finished products containing 25–30% fruit and citrus juices, 0.1–0.5% freeze-dried aromatic plants, 0.3–0.5% berry or vegetable powders, 0.3–0.6% spices were the objects of the research.
The results. It has been established that the use of cellulose and glycerin in the formulation of carbohydrate-fat binary compositions based on fruit, citrus juices, berry or vegetable powders and spicy-aromatic raw materials contributes to the formation of an elastic, delicate film structure and a complex of flavoring and aromatic substances, depending on the type of raw materials and VAD used. With an increase in the concentration of cellulose, the films become denser, and in combination with the main raw materials (juice, powders, spices), the amount of dry substances and extract content increase, the films become opaque and less elastic. It has been found that in all samples, with an increase in spicy-aromatic raw materials, the number of inclusions increases and the films acquire a mosaic appearance. For clarity of experiments, graphs of the dependence of the type of main raw material and VAD on the concentration of the recipe composition and the formation of the flavor and aromatic range have been presented.
Conclusion. The experimental data and dependencies obtained will be useful when considering more complex processes of mass transfer, sucrose inversion, and destruction of protein-carotenoid complexes. The perception of flavor and density of edible films is complex: the balance between sweetness, extractivity and threshold values of sensory characteristics are the main criteria for the organoleptic quality of the films being developed. It increases with the concentration of VAD and the type of juices or powders used. A richer and more pronounced taste appears.
The article presents the research on the adaptation and implementation of a method for determining mono- and diacylglycerols in vegetable oils. The relevance of the research is determined by the introduction of standardization of monochloropropanediols and glycidol in vegetable oils and some types of fat and oil products since 2025. According to the scientific literature, mono- and diacylglycerols, formed during the ripening of oil seeds under the influence of the enzyme lipase, are precursors of monochloropropanediols and glycidol, which, in turn, according to the World Health Organization, are carcinogenic substances. Some of the most probable mechanisms for the formation of contaminants from mono- and diacylglycerols have been considered; according to them diacylglycerols are considered to be the main source of glycidyl ethers, and monoacylglycerols are that of monochloropropanediols. An important factor influencing the speed and completeness of the described reactions is the presence in the system of water molecules, chlorine ions, hydrogen protons, as well as high temperatures. Catalysts for such side reactions are heavy metals, including their salts. Thus, in order to implement effective measures to control the formation of contaminants in oil, it is important to have a complete picture of the quality indicators of the feedstock and, above all, data on the content of mono- and diacylglycerols, methods for determining which are not available in laboratory practice in the Russian Federation.
The goal of the research is to develop a method for the quantitative determination of mono- and diacylglycerols in vegetable oils.
As a result of the research, the sample preparation method has been improved, calibration dependencies have been determined using an internal standard, and an identification and quantitative analysis technique has been proposed, on the basis of gas chromatography with a flame ionization detector and the use of a high-temperature capillary column. Verification work has been carried out and the metrological characteristics of the proposed method have been determined. The method has been tested on real objects – various types of vegetable oils.
The article studies the amino acid composition of the developed biscuit semi-finished products. When developing the recipe for gluten-free products, special attention was paid to the protein composition of the raw materials and the calculation of the amino acid score, since the lack of a certain amino acid affects the ability of gluten formation during the kneading process. In addition, an unbalanced amino acid composition can cause an imbalance in the human body and negatively affect the functioning of muscles, heart, brain and other organs, leading to diseases. The objectives of the research included the following: determination of the amino acid composition of gluten-free biscuit semi-finished products; analysis of amino acid composition and calculation of the amino acid score of gluten-free biscuit products. The amino acid composition was calculated according to well-known methods, taking into account the chemical composition of food products. During the calculation, the biological value of proteins was assessed, the utility coefficient, the amino acid composition balance coefficient, and the amino acid score difference coefficient were determined. It was established that the developed gluten-free biscuit contained a significant amount of threonine, namely 11.38 g per 100 g of protein. As a result of calculations, it was determined that the first limiting amino acid was tryptophan, the second was the compound «phenylalanine + tyrosine», the shares of which in the amino acid score were 0.12 and 0.90, respectively. An assessment of the complex balance of a gluten-free biscuit semi-finished product showed an insufficient level of balance in human nutrition, but the indicators of individual amino acids compared to the control made from wheat flour were higher, for example, valine by 1.8 times, isoleucine, leucine and lysine by 1.1 times, metheonine + cysteine 2.7 times and threonine 2.8 times.
Carotenes, which simultaneously have the properties of natural dyes and biologically active substances that have a positive effect on human health and life expectancy, are becoming increasingly widespread in the food, pharmacological and cosmetic industries. This necessitates an increase in the production of carotenes on an industrial scale. For use in food technologies, an encapsulated form of carotenes is recommended, which requires their high purity. The purpose of this study is to analyze domestic and foreign scientific, technical and patent information on existing methods for obtaining carotenes from carotenoid concentrates to determine the direction of research for the development of technology for the production of carotenes, in particular lycopene, ensuring the production of high purity lycopene from carotenoid concentrates, as well as increasing release of lycopene. The issues of using chromatographic methods for isolating lycopene, their advantages and disadvantages, as well as the use of non-polar and low-polar organic solvents for isolating lycopene from carotenoid concentrate are considered. The promising direction of research on the use of adsorption resins in the isolation of lycopene from carotenoid concentrates is noted.
It has been concluded that the currently existing methods for obtaining carotenes from carotenoid concentrates, in particular lycopene, are quite technically complex and time-consuming. Some of the mallow the production of high purity lycopene, but the yield of lycopene is so low that their industrial scaling seems impractical. Taking this into account, research is being updated in the field of developing a technology for producing lycopene from a carotenoid concentrate, providing a high yield of high purity lycopene, with the possibility of its implementation on an industrial scale.
The quality and safety of food products is one of the main priorities of the Russian State policy in the field of nutrition and well-being of the population.
Grape wines are a special type of food product; they are alcoholic beverages obtained as a result of complete or incomplete fermentation of whole or crushed fresh grapes or grape must. The combination of mutual influence of such factors as grape variety, climatic conditions, cultivation methods and production technology gives each wine its uniqueness and character.
These features are characteristic for natural drinks, which, unfortunately, are becoming fewer and fewer today, despite the implementation of control and supervisory activities and the adoption of various government regulation measures.
The presence of wines of dubious quality on the shelves gives rise to a number of serious consequences, both for public health and for the state economy.
The article presents the results of research on a comprehensive assessment of the quality of wines sold on the regional consumer market.
The determination of the organoleptic and physico-chemical parameters of wines regulated by GOST has been carried out according to the methods established in the current regulatory documents; the determination of the qualitative and quantitative composition of volatile components and cationic composition has been carried out using the gas chromatographic method and the capillary electrophoresis method.
It has been established that 30% of the total volume of wine samples studied is distinguished by rather low organoleptic characteristics, with full compliance with the established requirements in terms of physical and chemical indicators, and 10%, based on the total assessment of organoleptic, physical and chemical indicators, component and cationic composition, are identified as counterfeit products.
At present, «Strategy for Improving the Quality of Food Products in the Russian Federation until 2030» is the main document in the field of healthy nutrition. According to it, the main direction in this area is to provide the Russian population with nutritious food products, which will ensure the prevention of nutritional diseases and lead to to increase the life expectancy of the population, as well as improve their quality of life and to stimulate the production of quality products. At the same time, the need to use domestically produced raw materials, as well as functional ingredients from these raw materials in the production of a wide range of products for healthy and preventive nutrition, is clear lystated. The importance of using local plant, fruit and other raw materials, which are a source of many deficient vitamins, minerals, insoluble and soluble dietary fiber, as well as other valuable nutrients should be noted. In addition, local fruit raw materials are climatically adapted to the living conditions of humans in a particular region. In this regard, research aimed at analyzing the nutritional value of common regional fruit raw materials of the Central Chernozem Region (CCHR) and other regions of Russia is necessary. Of particular importance in the study of the nutritional value of berries and fruits as multivitamin crops that have a complex of medicinal and preventive properties. Among them are, undoubtedly, garden strawberries. The goal of the research is to study the consumer qualities and nutritional value of new varieties of garden strawberries. The article presents the results of a comprehensive assessment of the consumer qualities of the berries of seven new, currently zoned varieties of garden strawberries of foreign selection grown in the Tambov region: Aprika, Vivara, Zephyr, Kwiki, Letitia, Lord and Sibilla. The quality of berry raw materials is determined by a combination of organoleptic and physicochemical indicators, nutritional value, as well as safety indicators. The results of organoleptic studies showed that the berries of all studied varieties had excellent taste. At the same time, the berries of the Aprica, Kwiki, Zephyr and Vivara varieties had excellent quality. These fruits are classified as having an excellent quality. The berries of Laetitia, Sibylla and Lord varieties have been classified in the first quality category due to their good quality. Studies of the nutritional value of berries have shown that the berries of the Laetitia variety are leading in terms of ascorbic acid content, and the berries of the Zephyr variety in terms of anthocyanins. In terms of safety indicators, the berries of all studied varieties of garden strawberries meet the requirements of TR CU 021/2011 «On the safety of food products».
Improving the quality of food products is one of the strategic development goals of the Russian Federation. Its solution is possible through the production of new generation food products that can provide optimal nutrition and disease prevention. For a long time, the market for functiona land specialized products was represented mainly by imported products, therefore, under the sanctions, in order to eliminate import dependence, it is necessary to increase the range of domestic products in this segment. An analysis of consumer demand for gluten-free flour confectionery products (GFC) has shown that there is a shortage of such products in the food market. In connection with this, there is a need to conduct research on the development of new recipes and food technologies for people suffering from celiac disease. Therefore, the goal of the research is to substantiate the choice of gluten-free raw materials and to develop cupcakes using hydrocolloids. Samples of gluten-free flour (rice, flax, millet), gluten-free flour mixtures and gluten-free muffins were the objects of the research. As a result of comprehensive research, a gluten-free mixture with a ratio of rice, flaxseed, millet flour (50:25:25) has been identified, which satisfies the requirements for kneading gluten-free dough. The optimal dosage of 2.0% apple pectin when kneading dough has been established, which has made it possible to prepare gluten-free muffins that are not inferior in quality to the traditional Stolichny muffin made from wheat flour.
The industry of bakery products that do not contain gluten has not been studied enough, so this topic is gaining popularity among scientists. The subject of the study was the development of a bread recipe using almond flour. Pumpkin puree and dried seaweed Laminaria ochroleuca were selected as additives. To improve the taste characteristics of the product, it was decided to add dietary fiber, which can add value to the finished product. The goal of the work was to develop a bread recipe using almond flour. The research was carried out in several stages: 1. development of a recipe and production of prototypes;2. analysis of organoleptic indicators; 3. carrying out physical and chemical studies (determination of humidity, acidity). To carry out the analysis, instrumental and visual research methods were used. During the experiment, five samples of bread were obtained, four of them with the addition of different concentrations of pumpkin and seaweed puree. The rate of dietary fiber was calculated based on the daily consumption rate. Sample No. 2, made with the addition of pumpkin puree in an amount of 100 g, had the best organoleptic properties. The bread had a pleasant taste and smell characteristic of the added raw materials, a soft and baked crumb, and the presence of pores. Samples of bread with added seaweed had a specific taste and smell, and traces of unkneading. The research results can be used in the baking industry to expand the range of bakery products. The developed bread can become a very useful product, especially for those categories of the population who are deficient in certain components. Also, given that for the majority of the population bread is a commodity of everyday demand, its preparation will be in great demand.
Extension of the assortment of products with increased nutritional and biological value can be achieved in different ways. One way is to use various supplements containing functional ingredients. This approach makes it possible to create food products with specified functional properties. This article presents a developed flour confectionery product, i.e. butter cookies using various types of flour and flax seeds. The characteristics of the chemical composition and functional properties of flax seeds have been presented. The choice of flax seeds as a functional ingredient is due to their high content of biologically active substances, a sufficiently large raw material base and availability. The use of flax seeds in the technology of butter cookies to enrich them with vitamins and minerals has been substantiated. Flax seeds and their processed products have a unique chemical composition, a wide range of properties and a set of biologically active substances. The influence of oatmeal and flaxseed flour on the quality of butter cookies has been studied and their optimal dosages in the cookie recipe selected. The optimal percentage ratio of wheat and oat flour has been established –90:10; wheat and flax flour – 90:10. The influence of flax seed dosage on the quality of butter cookies has been studied and the optimal percentage of flax seeds established – 10%. A recipe and technology for three types of butter cookies have been developed: butter cookies with the addition of flax seeds, butter cookies with the addition of oatmeal and flax seeds, butter cookies with the addition of flax flour and flax seeds.
AGRICULTURAL SCIENCES
The article studies the influence of soil carbonates on the plants, the yield and the quality of highbush blueberries in the conditions of the Kabardino-Balkarian Republic. The carbonates in the soil solution lead to an increase in soil pH, which negatively affects its development. High bush blueberries have special soil requirements for normal growth and development. It is necessary to provide the plant with a breathable substrate with an optimal acidity level of up to 5.5 units. Acidic conditions are necessary due to the unique structure of the root system: highbush blueberries are completely devoid of root hairs; their function is performed by a colony of fungi, which constitutes mycorrhiza in symbiosis with the plant. Mycorrhiza functions only in acidic conditions. The research provides average data for the period of 2021-2023. Blueberry plants grown in carbonate areas are characterized by weak growth, few shoots to form, low foliage and chlorosis due to reduced food availability. The size of the berries and their taste play a huge role for producers. The measurement of this parameter showed that in the main part of the field, under the best growing conditions, the average diameter of the berries is 17 mm, which is 23.5% more than in carbonate areas, where the diameter is, on average, 13 mm. The accumulation of sugar in berries with increased soil carbonate decreases by2.3%, which significantly affects their taste. As a result of the research, a negative effect of soil carbonates on the development of blueberry plants has been noted, as well as yield reduction by 79.5%. The negative effect of increasing pH to neutral or, especially, slightly alkaline on plant development is associated with deterioration of nutrition and poor development of the root system.
The goal of the research was to develop a technology for introducing Salicornia europaea L.into in vitro culture. Methods of cultivating meristems and callus cultures were studied. To cultivate meristems, the tip of the apical bud were used as an explant. Fragments of stems and leaves were used toobtain callus tissue. To study the influence of various factors on germination, seeds were soaked in sterile tap water and in solutions of gibberellin, cytokinin, auxin and NaCl, and were also subjected to cold stratification (independently and with subsequent placement in Knop’s agarized medium). Salicornia seeds were sterilized with various antiseptics: 70% alcohol, 10% aqueous solution of sodium hypochlorite («Belizna»), amoxicillinand 3% hydrogen peroxide. The ability of the culture to form callus was studied in MS medium. As a result, it was determined that the highest germination of seeds was observed in Knop medium after treating theseeds with a suspension of green algae of the Scenedesmus genus, as well as after preliminary cold stratification, and slightly less after treating the seeds with a solution of NaCl and gibberellic acid. The most effective method of seed sterilization turned out to be treatment with alcohol followed by treatment with sodium hypochlorite. A comparative analysis of seed germination in filter paper in Petri dishes, Knop agar medium, Murashige and Skoog (hormone-free) was carried out. The ability of S. europaea L. to form callusin MS medium with phytohormones was assessed. Conclusion. To improve germination, it is recommended to subject the seeds to cold stratification. To obtain aseptic explants quickly, it is recommended to germinate the seeds in the Knop nutrient medium, having previously sterilized them with alcohol, then with sodiumhypochlorite, followed by washing with distilled water. The most suitable type of microclonal propagationfor S. europaea L. is the production of callus tissue followed by induction of organogenesis or embryogenesis.
Walnut (Juglans regia L.) is a particularly valuable plant for humans. This is due to its multifaceted beneficial properties and qualities as a food, medicinal, technical, recreational and environmental tree species. The main goal of nut breeding is to obtain high-quality fruits. In the Russian Federation for the selection of individuals that provide such products, two methods are mainly used: method 1 – «Program and method for breeding walnuts» (Sukhorukikh et al., 2007) and method 2 – «Program and method for the study of fruit, berry and nut crops» (1999). The purpose of the study is to study the diversity of promising forms of walnut identified on the basis of methods 1 and 2 in terms of taste, kernel extractability and the overall score of the breeding value (quality) of the fruit. Other characteristics assessed identically or similarly are not considered. The object of the research was the literature data of the authors assessing the quality of nuts using methods 1, 2. For statistical processing, the Stadia 8.0/prof. program was used, and Microsoft Excel was used for graphical construction. Diversity was assessed using coefficients of variation and indices of distributed rank diversity (IRRR) based on known methods. It was established that the coefficients of variation of the studied indicators, assessed using various methods, did not have a significant statistical difference, and the selection gradations of taste and kernel extractability were equally distributed. When assessed using method 1, the statistical distribution of the total score of the selection value of fruits had a normal distribution, when using method 2 it differed from normal. When assessing diversity using IRRR values, method 1, compared to method 2, turned out to be more sensitive and provided greater diversity of the allocated gene pool in terms of the overall score of the breeding value of walnut fruits. The results can be used in walnut breeding.
ISSN 2713-0029 (Online)