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Chromatographic determination of vitamin content in a bar with dried tomatoes

https://doi.org/10.47370/2072-0920-2024-20-1-35-41

Abstract

There exists a problem of unbalanced nutrition in the modern world. Consumption of vegetables and fruits is mainly seasonal and they are not consumed by everyone in the daily diet in sufficient quantities to meet the daily requirement for nutrients. Snack products, including various bars, are becoming increasingly popular among the population of our country. Often there is an insufficient amount of vitamins and other micronutrients with such products that are so necessary to ensure the health of the human body. Insufficiency of these nutrients causes various diseases, the condition of the skin, hair, nails, and even mood worsens.

The goal of the research was to determine the amount of vitamins in a bar made from sunflower seeds with the addition of plant materials – crushed dried tomato fruits.

The objects of the study were a candy bar with the addition of rusk crumbs, containing 15% of dried tomatoes by weight of the finished product and a control sample – kozinak.

Tests were carried out on a Dionex Ultimate 3000 chromatograph using reverse phase chromatography on a Luna C18 column. This device is used to measure the content of a wide range of components in samples of substances and materials, solutions, food products and other objects of analysis.

It has been found that with the addition of a herbal additive and cracker crumbs to the recipe, the quanitative composition of vitamins A and E in the finished product increased compared to the control sample.

The results of the research can be used in the development of new food recipes.

About the Authors

V. A. Bukhovets
Federal State Budgetary Educational Institution of Higher Education «Saratov State University of Genetics, Biotechnology and Engineering named after N.I. Vavilova»
Russian Federation

Valentina A. Bukhovets, PhD (Eng.), Associate Professor

Petr Stolypin prospect, building 4, suite 3, 410012, Saratov

tel.: +7 (927) 624 54 80



N. A. Semilet
Federal State Budgetary Educational Institution of Higher Education «Saratov State University of Genetics, Biotechnology and Engineering named after N.I. Vavilova»
Russian Federation

Nikita A. Semilet, PhD (Eng.), Associate Professor

Petr Stolypin prospect, building 4, suite 3, 410012, Saratov



Yu. D. Novikova
Federal State Budgetary Educational Institution of Higher Education «Saratov State University of Genetics, Biotechnology and Engineering named after N.I. Vavilova»
Russian Federation

Yulia D. Novikova, Master student

Petr Stolypin prospect, building 4, suite 3, 410012, Saratov



References

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2. Kodentsova V.M., Pogozheva A.V., Gromova O.A. et al. Vitamin-mineral complexes in the nutrition of the adult population. Nutrition issues. 2015; 6. [in Russian]

3. Shelemetyeva O.V. Determination of vitamins by high-performance liquid chromatography in premixes, dietary supplements and food products: dis. ...PhD (Chem.). M.; 2009. [in Russian]

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5. Krolevets A.A., Myachikova N.I., Binkovskaya O.V. et al. Nanostructured vitamin E: its properties and application in functional foods. Commodity specialist of food products. 2021; 5: 372-381. [in Russian]

6. GOST EN 12822-2020. Food products. Determination of vitamin E content by high-performance liquid chromatography. Measurement of alpha, beta, gamma and delta tocopherols. M.: RST; 2022. [in Russian]

7. GOST EN 12823-2-2014. Food products. Determination of vitamin A content by high-performance liquid chromatography. M.: Standartinform; 2014. [in Russian]

8. Shapovalova E.N., Pirogov A.V. Chromatographic methods of analysis: a manual for a special course. M.: Moscow State University named after. M.V. Lomonosov; 2010. [in Russian]


Review

For citations:


Bukhovets V.A., Semilet N.A., Novikova Yu.D. Chromatographic determination of vitamin content in a bar with dried tomatoes. New Technologies. 2024;20(1):35-41. (In Russ.) https://doi.org/10.47370/2072-0920-2024-20-1-35-41

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ISSN 2072-0920 (Print)
ISSN 2713-0029 (Online)