Modern research on the extraction of biologically active substances from plant raw materials using enzymes

The article overviews modern studies of domestic and foreign scientists in the field of extraction of biologically active substances from plant materials using enzymes. Extraction of biologically active substances using enzymes allows for controlled biotransformation of plant materials due to the precise specificity and selectivity of enzymes, which creates mild conditions that ensure the preservation of biologically active substances. It has been noted that the quality of the extractant used has a significant effect on the efficiency of the extraction process. Thus, raw materials treated with a combination of an electrolyte and an enzyme are extracted more efficiently by reducing the mass transfer barrier. The main advantage of using supercritical fluids as an extractant is a decrease in temperature or pressure that leads to the precipitation of the extractable substance. One of the main disadvantages of the process of extracting biologically active substances from plant materials using enzymes is their high cost. To eliminate this disadvantage, the following intensification methods are used: ultrasonic treatment, high pressure treatment and microwave treat- ment. The most promising methods for intensifying the process of extracting biologically active substances from plant materials using enzymes are methods of ultrasonic and microwave treatment. Ultrasonic treatment under optimal conditions allows increasing the activity of enzymes, and microwave treatment provides more efficient penetration of the extractant into the tissues of plant material, due to the destruction of cell walls.

1. Modern technologies of functional food products / Ed. by A.B. Lisitsin, V.N. Ivanova. M.: DeLi plus. 2018. 432 p.

2. Puri M., Sharma D., Barrow C.J. Enzyme assisted extraction of bioactives from plants // Trends in Biotechnology. 2012. № 30 (1). P. 37-44. DOI: 10.1016/j.tibtech.2011.06.014.

3. Shamraja S. Nadar, Priyanka Rao, Virendra K. Rathod. Enzyme assisted extraction of biomolecules as an approach to novel extraction technology // Food Research International. 2018. P. 309–330. DOI: 10.1016/j.foodres.2018.03.006

4. Extraction and characterisation of pomace pectin from gold kiwifruit (Actinidia chinensis) / Yuliarti O. [et al.] // Food Chemistry. 2015. P. 290–296. DOI: 10.1016/j.foodchem.2015.03.148.

5. Application of non-conventional extraction methods: toward a sustainable and green production of valuable compounds from mushrooms / E. Rosello-Soto [et al.] // Food Engineering Reviews. 2016. № 8 (2). P. 214–234. DOI: 10.1007/s12393-015-9131-1.

6. Comparison of acidic and enzymatic pectin extraction from passion fruit peels and its gel properties / Liew S. Q. [et al.] // Journal of Food Process Engineering. 2016. № 39 (5). P. 501–511. DOI: 10.1111/jfpe.12243.

7. Application of enzymes for efficient extraction, modification, and development of functional properties of lime pectin / M. Dominiak [et al.] // Food Hydrocolloids. 2014. № 40. P. 273-282. DOI: 10.1016/j.foodhyd.2014.03.009.

8. New Biotechnology for the Production of Phytocompounds from Secondary Products of Grain Processing / Bityukova A.V. [et al.] // Food Processing: Techniques and Technology. 2019. Vol. 49, № 1. P. 11–13. DOI: 10.21603/2074-9414-2019-1-5-13.

9. Adadi P., Filippova D.S., Barakova N.V. The effect of enzyme preparations on extracting the pigments from plant raw materials // Vestnik Mezhdunarodnoi akademii kholoda. 2019. No 1. P. 64–68. DOI: 10.17586/1606-4313-2019-18-1-64-68.

10. Bonareva V.K., Khabibulina N.V., Krasnoshtanova A.A. Study of the effect of enzymatic treatment on the carbohydrate component of soybean molasses of a new type // Advances in chemistry and chemical technology. 2019. Vol. 33, No. 5. P. 28–30.

11. Application of ionic liquids based enzyme assisted extraction of chlorogenic acid from Eucommia ulmoides leaves / Liu T. [et al.] // Analytica Chimica Acta. 2016. № 903. P. 91–99. DOI: 10.1016/j.aca.2015.11.029.

12. Microwave assisted extraction of essential oils from enzymatically pretreated lavender (Lavandula angustifolia Miller) / Calinescu I. [et al.] // Central European Journal of Chemistry. 2014. № 12 (8). P. 829–836. DOI: 10.1016/j.cep.2017.02.003.

13. Extraction of phytochemicals from saffron by supercritical carbon dioxide with water and methanol as entrainer / Nerome H. [et al.] // The Journal of Supercritical Fluids. 2016. № 107. P. 377–383. DOI: 10.1016/j.supflu.2015.10.007.

14. Shitu A., Izhar S., Tahir T.M. Sub-critical water as a green solvent for production of valuable materials from agricultural waste biomass: A review of recent work // Global Journal of Environmental Science Management. 2015. № 1 (13). P. 255–264. DOI: 10.7508/gjesm.2015.03.008.

15. Wang X., Chen Q., Lu X. Pectin extracted from apple pomace and citrus peel by subcritical water // Food Hydrocolloids. 2014. № 8. P. 129–137. DOI: 10.1016/j.foodhyd.2013.12.003.

16. Enzyme assisted supercritical fluid extraction: An alternative and green technology for nonextractable polyphenols / Mushtaq M. [et al.] // Analytical and Bioanalytical Chemistry. 2017. № 409 (14). P. 3645–3655. DOI: 10.1007/s00216-017-0309-7.

17. Rao P.R., Rathod V.K. Mapping study of an ultrasonic bath for the extraction of andrographolide from Andrographis paniculata using ultrasound // Industrial Crops and Products. 2015. P. 312–318. DOI: 10.1016/j.indcrop.2014.11.046.

18. Sojitra U.V., Nada, S.S., Rathod V.K. Immobilization of pectinase onto chitosan magnetic nanoparticles by macromolecular cross-linker // Carbohydrate Polymers. 2017. P. 677–685. DOI: 10.1016/j.carbpol.2016.10.018.

19. Enzymolysis-ultrasonic assisted extraction, chemical characteristics and bioactivities of polysaccharides from corn silk / Chen S. [et al.] // Carbohydrate Polymers. 2014. № 101 (1). P. 332– 341. DOI: 10.1016/j.carbpol.2013.09.046.

20. Efficient extraction of pectin from sisal waste by combined enzymatic and ultrasonic process / Yishuo Yang [et al.] // Food Hydrocolloids. 2018. Vol. 79. P. 189-196. DOI: 10.1016/j. foodhyd.2017.11.051.

21. Velyamov Sh.M., Jingilbaev S.S. Study of the Effect of Active Mixing of the Extractant on the Yield of Pectin During Enzymatic Extraction from Beetroot // Bulletin of the South Ural State University. Seria: Food and Biotechnology. 2020. Vol. 8, No. 1. P. 40–48. DOI: 10.14529/ food200105.

22. Extraction of anthocyanins from grape by-products assisted by ultrasonics, high hydrostatic pressure or pulsed electric fields: A comparison / Corrales M. [et al.] // Innovative Food Science & Emerging Technologies. 2008. № 9 (1). P. 85–91. DOI: 10.1016/j.ifset.2007.06.002.

23. Effects of high pressure extraction on the extraction yield, total phenolic content and antioxidant activity of longan fruit pericarp / Prasad K.N. [et al.] // Innovative Food Science & Emerging Technologies. 2009. № 10 (2). P. 155–159. DOI: 10.1016/j.ifset.2008.11.007.

24. Shouqin Z., Jun X., Changzheng W. High hydrostatic pressure extraction of flavonoids from propolis // Journal of Chemical Technology & Biotechnology. 2005. № 80 (1). P. 50–54. DOI: 10.1002/ jctb.1153.

25. Saifuddin N., Saltanat A., Refal H. Enhancing the removal of phenolic compounds from palm oil mill effluent by enzymatic pretreatment and microwave-assisted extraction // Chemical Science Transactions. 2014. № 3 (3). P. 1083–1093.

26. Microwave-assisted extractions of active ingredients from plants / Chan C. [et al.] // Journal of Chromatography A. 2011. № 1218 (37). P. 6213–6225. DOI: 10.1016/j.chroma.2011.07.040.

27. Chanioti S., Siamandoura P., Tzia C. Evaluation of extracts prepared from olive oil byproducts using microwave-assisted enzymatic extraction: Effect of encapsulationт on the stability of final products // Waste and Biomass Valorization. 2016. № 7 (4). P. 831–842.

28. Enzyme assisted extraction of carbohydrates from the brown alga Ecklonia radiata: Effect of enzyme type, pH and buffer on sugar yield and molecular weight profiles / Charoensiddhi S. [et al.] // Process Biochemistry. 2016. № 51 (10). P. 1503-1510. DOI: 10.1016/j.procbio.2016.07.014.

29. Optimisation of microwave-assisted enzymatic extraction of corilagin and geraniin from Geranium sibiricum Linne and evaluation of antioxidant activity / Yang Y.C. [et al.] // Food Chemistry. 2010. № 122 (1). P. 373-380. DOI: 10.1016/j.foodchem.2010.02.061.