SELECTION FOR DRY RESISTANCE OF SOMATIC CLONES OF TEA PLANTS (CAMELLIA SINENSIS (L.) O. KUNTZE) IN IN VITRO CULTURE
https://doi.org/10.24411/2072-0920-2020-10313
Abstract
The article presents research materials on the selection of in vitro drought tolerant somatic tea clones (Sc-4, Sc-15, Sc-27, Sc-33) obtained by induction of hemogenesis from callus tissue. Osmotic stress was modeled by adding polyethylene glycol (PEG-30.0 g/l) to the growth medium. Changes in physiological parameters during water deficiency were studied by the relative electrical conductivity and stability of cell membranes using a ST300C conductometer.
The addition of PEG to the nutrient medium caused an increase in electrical conductivity in all studied somatic tea clones, with the exception of Sc-27, in which this indicator was lower compared to the control one.
The stability of cell membranes in Sc-27 was higher compared to the control one. These data indicate a low level of damage to plant tissue. PCR analysis showed that the dehydrin 2 (DHN2) gene, one of the main genetic markers of drought response of plants, was expressed significantly higher in somaclone Sc-27. For all the parameters studied the Sc-27 somatic tea clone can be preliminarily selected as a drought tolerant genotype and can be used in a further breeding program.
The addition of PEG to the nutrient medium caused an increase in electrical conductivity in all studied somatic tea clones, with the exception of Sc-27, in which this indicator was lower compared to the control one.
The stability of cell membranes in Sc-27 was higher compared to the control one. These data indicate a low level of damage to plant tissue. PCR analysis showed that the dehydrin 2 (DHN2) gene, one of the main genetic markers of drought response of plants, was expressed significantly higher in somaclone Sc-27. For all the parameters studied the Sc-27 somatic tea clone can be preliminarily selected as a drought tolerant genotype and can be used in a further breeding program.
About the Author
M. V. Gvasaliya
Federal State Budgetary Institution of Science (Federal Research Center of the Subtropical Scientific Center of the Russian Academy of Sciences)
Russian Federation
Russian Federation
Candidate of Biology, a senior researcher of the Department of Biotechnology
Sochi
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Review
For citations:
Gvasaliya M.V. SELECTION FOR DRY RESISTANCE OF SOMATIC CLONES OF TEA PLANTS (CAMELLIA SINENSIS (L.) O. KUNTZE) IN IN VITRO CULTURE. New Technologies. 2020;(3):114-121. (In Russ.) https://doi.org/10.24411/2072-0920-2020-10313
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