RESEARCH OF MICROBIOLOGICAL SYNTHESIS OF BIOLOGICALLY ACTIVE SUBSTANCES IN SOLUTIONS OF BEET MOLASSES
https://doi.org/10.24411/2072-0920-2020-10207
Abstract
Treatment of solutions of beet molasses with 5 strains of microorganisms belonging to 4 species in order to enrich the solutions with β-carotene, vitamin B2 and organic acids have been studied. The influence of the initial pH value, as well as the optimal dry matter content in molasses solutions, which ensure the effective vital activity of microorganisms with the synthesis of biologically active substances, has been revealed. It has been found that F-3758 and F-3759 Blakeslea trispora strains in molasses solutions are not capable of synthesizing β-carotene.
It has been found that B-501 Bacillus subtilis strain exhibits high activity in molasses solutions with initial pH values of 7.0 and 8.5 and a dry matter content of not more than 20 %. As a result of life activity in molasses solutions of Bacillus subtilis, strain B-501 synthesizes vitamin B2 in the amount of 1.42-1.93 mg/g of sugars. It has been revealed that Y-2482 Debaryomyces hansenii strain in molasses solution with an initial pH of 7.0 synthesizes organic acids in an amount of 1.790 (mmol/100 ml)/g of sugars. In addition, Y-2482 Debaryomyces hansenii strain is capable of vital activity in solution with an initial pH of 8.5, but some of its vital products are neutralized. It has been established that Y-2305 Guehomyces pullulans is capable of vital activity in molasses solutions, however, its vital products are not only organic acids, but also some compounds with oxy groups, as a result of which they are neutralized. Ultimately, the amount of synthesized Y-2305 Guehomyces pullulans organic acids is 0.2 (mmol/100 ml)/g of sugar. It has been proved that B-501 Bacillus subtilis strain and Y-2482 Debaryomyces hansenii strain are promising strains for enriching beet molasses solutions with biologically active substances.
It has been found that B-501 Bacillus subtilis strain exhibits high activity in molasses solutions with initial pH values of 7.0 and 8.5 and a dry matter content of not more than 20 %. As a result of life activity in molasses solutions of Bacillus subtilis, strain B-501 synthesizes vitamin B2 in the amount of 1.42-1.93 mg/g of sugars. It has been revealed that Y-2482 Debaryomyces hansenii strain in molasses solution with an initial pH of 7.0 synthesizes organic acids in an amount of 1.790 (mmol/100 ml)/g of sugars. In addition, Y-2482 Debaryomyces hansenii strain is capable of vital activity in solution with an initial pH of 8.5, but some of its vital products are neutralized. It has been established that Y-2305 Guehomyces pullulans is capable of vital activity in molasses solutions, however, its vital products are not only organic acids, but also some compounds with oxy groups, as a result of which they are neutralized. Ultimately, the amount of synthesized Y-2305 Guehomyces pullulans organic acids is 0.2 (mmol/100 ml)/g of sugar. It has been proved that B-501 Bacillus subtilis strain and Y-2482 Debaryomyces hansenii strain are promising strains for enriching beet molasses solutions with biologically active substances.
Supporting agencies: The research has been carried out within Grant No. 19-416-233002 “Identification of patterns of influence of microbiological processing of beet molasses on the composition and content of biologically active substances of the obtained products” with the support of the RFPF and the Administration of the Krasnodar Territory.
About the Authors
S. O. Semenikhin
Krasnodar SRI for Storage and Processing of Agricultural Products – a branch of the FSBNU «The North Caucasian Federal Scientific Center for Horticulture, Viticulture, Winemaking» (KSRICHI – a branch of the FSBSI NCFSCHVW)
Russian Federation
Candidate of Technical Sciences, a senior researcher of the Department of Technology of Sugar and Sugar Products
Russian Federation
Candidate of Technical Sciences, a senior researcher of the Department of Technology of Sugar and Sugar Products
M. V. Babakina
Krasnodar SRI for Storage and Processing of Agricultural Products – a branch of the FSBNU «The North Caucasian Federal Scientific Center for Horticulture, Viticulture, Winemaking» (KSRICHI – a branch of the FSBSI NCFSCHVW)
Russian Federation
a junior researcher of the Department of Storage and Complex Processing of Agricultural Raw Materials
Russian Federation
a junior researcher of the Department of Storage and Complex Processing of Agricultural Raw Materials
O. V. Fedoseeva
Krasnodar SRI for Storage and Processing of Agricultural Products – a branch of the FSBNU «The North Caucasian Federal Scientific Center for Horticulture, Viticulture, Winemaking» (KSRICHI – a branch of the FSBSI NCFSCHVW)
Russian Federation
a junior researcher of the Department of Food Technology, Quality Control and Standardization
Russian Federation
a junior researcher of the Department of Food Technology, Quality Control and Standardization
V. O. Gorodetsky
Krasnodar SRI for Storage and Processing of Agricultural Products – a branch of the FSBNU «The North Caucasian Federal Scientific Center for Horticulture, Viticulture, Winemaking» (KSRICHI – a branch of the FSBSI NCFSCHVW)
Russian Federation
Candidate of Technical Sciences, head of the Department of Sugar and Sugar Products Technology
Russian Federation
Candidate of Technical Sciences, head of the Department of Sugar and Sugar Products Technology
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Review
For citations:
Semenikhin S.O., Babakina M.V., Fedoseeva O.V., Gorodetsky V.O. RESEARCH OF MICROBIOLOGICAL SYNTHESIS OF BIOLOGICALLY ACTIVE SUBSTANCES IN SOLUTIONS OF BEET MOLASSES. New Technologies. 2020;(2):68-79. (In Russ.) https://doi.org/10.24411/2072-0920-2020-10207
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